Chemical Analysis & Separation

We bring years of experience and expertise in analysing and separating small molecules and peptides for various type of applications and industries.​

Using our well-equipped liquid chromatography (HPLC), mass spectroscopy (MS) and nuclear magnetic resonance (NMR) instrument park, our dedicated team efficiently performs chemical analysis and separation of organic compounds, peptides, and several other type of molecules at various detail levels.  Besides standard compound quality control (purity and/or identity), we offer in-depth analyses of unidentified molecules, low level impurities and unknown mixtures. 

Purity analysis by HPLC-UV/MS

Evaluation of compound purity by HPLC-UV/MS.

Typical Analytes:

Organic molecules, natural products, and peptides.

Delivered Result:

Relative purity plus detected mass of major compound.

 

Confirmation of identity by MS 

Confirmation of compound identity by high-resolution mass spectrometry (LC-MS).

Typical Analytes:

Small organic molecules.

Delivered Result:

Detected mass and suggestion on elemental composition.

 

Analysis of impurities

Quantification and characterization impurities in samples by HPLC-UV/MS.

Typical Analytes:

Organic molecules, natural products, and peptides.

Delivered Result:

Relative amounts as estimated from the peak areas and detected mass of all resolved impurities.

 

Optional: High-resolution LC-MS  with proposed elemental composition.

 

Characterization of mixtures

Characterisation of mixtures by HPLC-UV/MS.

Typical Analytes:

Organic molecules, natural products, and peptides.

Delivered Result:

Relative amounts as estimated from the peak areas and detected mass of resolved molecular components.

 

Optional: High-resolution LC-MS with proposed elemental composition.

NMR data analysis: Structural elucidation by NMR of selected components isolated by preparative chromatography.

We offer services ranging from cost-efficient standard packages up to fully tailored studies. Different report formats are available. Two popular and powerful standard analytical packages for small organic molecules are described below. Please contact us to find out more.

Purity analysis & structure ID – normal

Purity and structure analysis by HPLC-UV/MS analysis and 1D 1H NMR – our standard package.

Typical Analytes:

Small organic molecules.

Delivered Result:

Written report containing:​

    • Relative purity and detected mass of major compound.​
    • Verification of chemical structure using NMR data.​
    • Brief summary of observed impurities.

Purity analysis & structure ID – premium

Purity and structure analysis by HPLC-UV/MS analysis, high-resolution MS, and NMR (1D 1H, 1D 13C, 2D COSY and 13C-1H HSQC).

Typical Analytes:

Small organic molecules.

Delivered Result:

Written report containing:

    • Relative purity and detected mass of major compound
    • Proposed elemental composition
    • High level verification of chemical structure using NMR data
    • Summary of observed impurities

Our expertise in preparative LC helps you solve your purification and separation tasks. We work with different types of organic compounds such as small molecules, natural products, peptides and other similar organic molecules.

Some examples of services below:

Purification of single compounds

Purification in milligram to gram scale.

Typical Analytes:

Organic molecules, natural products, and peptides.

Delivered Result:

Isolated compound returned  in requested format together with written report containing purification conditions and HPLC-UV/MS analysis of isolated compound.

 

Optional: Verification of chemical structure using NMR data.

 

Purification of compound libraries

Purification of compounds from same compound class in milligram scale.

Libraries containing tens to hundreds of compounds can be purified.

Typical Analytes:

Small organic molecules.

Delivered Result:

Isolated compounds returned in requested format together with written report containing purification conditions and HPLC-UV/MS analysis of isolated compounds.

 

Optional: Verification of chemical structure using NMR data.

 

Characterization of molecules in mixtures

Separation of mixtures of organic molecules, e.g. impurities and byproducts.

Isolated compounds analyzed by UV and MS.

Typical Analytes:

Organic molecules, natural products.

Delivered Result:

Isolated compounds are returned in requested format together with written report containing separation conditions, low-resolution HPLC-UV/MS and high-resolution MS of isolated compounds.

 

Optional: NMR data analysis: Structural elucidation by NMR of selected components.